Background: Osteoarthritis (OA) is the most common form of arthritis and a leading cause of disability. This study\nattempted to investigate the key mRNAs and miRNAs related to OA.\nPatients and methods: From April 17th, 2018 to May 17th, 2018, five patients with OA and three normal controls\nwere enrolled in this present study. To identify the differentially expressed mRNAs (DEmRNAs) and miRNAs\n(DEmiRNAs) between patients with OA and normal controls, RNA-sequencing was performed. Then, DEmiRNAtarget\nDEmRNAs analysis and functional annotation of DEmiRNA-target DEmRNAs were performed. To validate the\nRNA-sequencing results, quantitative real time-PCR (RT-PCR) and western blot analysis were performed as well.\nResults: A total of 1068 DEmRNAs, 21 DEmiRNAs and 395 DEmiRNA-DEmRNA pairs were identified in synovial\ntissues of patients with OA. The functional annotation of DEmiRNA-target DEmRNAs revealed that Pathways in\ncancer and PI3K-Akt signaling pathway were significantly enriched Kyoto Encyclopedia of Genes and Genomes\n(KEGG) pathways. QRT-PCR and western blot results revealed that except for TLR7, the expression level of the others\nwas consistent with the RNA-sequencing results, generally.\nConclusion: The findings of this present study may provide new clues for the roles of DEmRNAs and DEmiRNAs in\nthe pathogenesis of OA.
Loading....